BACKGROUND Sperm aster organization during bovine and human fertilization requires a paternally-derived centriole that must first disengage from the sperm tail connecting-piece. We investigated the participation of the 26S proteasome in this process. METHODS Proteasome localization and enzymatic activity were studied in normal and pathological human spermatozoa by immunocytochemistry and enzyme...

An experimental work dealing with the gene modification using Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into cytoplasm. Oocytes that extruded first second polar bodies used for injection. 2 series microinjections designs early bovine embryos made. The degeneration r...

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

The aim of this study was to investigate the effects of iron and copper on bovine oocyte maturation, preimplantation embryo development and apoptosis of blastocysts. The concentrations of iron in the culture media were 0 (control), 0.45, 0.81, 1.96 and 3.26 mg/l, and the concentrations of copper were 0 (control), 0.093, 0.27, 0.46 and 0.68 mg/l. The changes in the iron (1.96 mg/l) and copper co...

Retinoids are recognized as important regulators of vertebrate development, cell differentiation, and tissue function. Previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events, including follicular development, oocyte maturation and early embryonic development. The present study evaluated in vitro effects of retinol addition to media c...

The changes in epigenetic modifications during early embryonic development significantly impact mammalian genome activation (EGA) and are species-conserved to some degree. Here, we reanalyzed the published RNA-Seq of human, mouse, goat embryos found that Zfp296 (zinc finger protein 296) expression was higher at EGA stage than oocyte all three species (adjusted p-value < 0.05 |log2(foldchange...

background: the cells of mammalian female reproductive tract have been widely used for in vitro fertilization (ivf). this study was designed to study the effects of oviductal epithelial cells (oecs) and their conditioned medium during ivf on subsequent embryonic development and the relative abundance of zygote arrest 1 (zar1) transcript in ovine zygotes. methods: the in vitro matured ovine oocy...

Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were no...